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Item #:
19305547
Millipore UFC901024 Regenerated Cellulose Amicon Ultra Centrifugal Filter Unit with PLGC Ultracel-10 Membrane, 15mL Capacity, 10kDa NMWL, 29.7mm
Item #:
19305547
€ 309
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Order now and get it around Friday, July 17
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High recovery ultracel regenerated cellulose membrane in a range of molecular weight cut-offs; high retentate recovery of > 90 percent
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What Stands Out
High Capacity
The 15mL capacity allows for efficient processing of larger sample volumes, enhancing productivity in laboratory applications.
Precision Filtration
Utilizes PLGC Ultracel-10 membrane with a 10kDa NMWL, ensuring fine filtration that effectively separates molecules based on size for reliable results.
Robust Design
With durable construction and multiple units in a pack, these filters offer cost-effective solutions for repeated experiments without compromising quality or performance.
Product Details
- High retentate recovery of > 90 percent with ultracel regenerated cellulose membrane
- Vertical membrane reduces concentration polarization for spin times as fast as 10-15 minutes
- Heat-sealed membrane minimizes downstream extractables; 100 percent integrity tested
- Convenient sample monitoring with translucent housing and volume gradations
- Direct pipette sample access eliminates processing step to recover concentrate
- Key applications: protein purification, western blotting, protein extraction
| Item Weight | 2 lbs (910 grams) |
Who Should Buy?
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Research Laboratories
Ideal for academic and industrial labs needing efficient protein concentration and purification capabilities.
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Biotechnology Companies
Excellent for companies developing biopharmaceuticals requiring precise filtration with rigorous quality standards.
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Clinical Research
Useful for clinical studies looking to analyze small biomolecules or proteins in a controlled manner.
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Home Use
Not suitable for home environments due to the technical nature and specialized applications of the product.
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Small-Scale Projects
Less efficient for small projects needing minimal filtration, where larger filters could be overkill.
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Non-Scientific Use
Unsuitable for users outside scientific fields that do not require membrane filtration technology.
Product Description
Millipore UFC901024 Regenerated Cellulose Amicon Ultra Centrifugal Filter Unit with PLGC Ultracel-10 Membrane, 15mL Capacity, 10kDa NMWL, 29.7mm Diameter x 122mm Length, 7.6 sq cm Membrane (Pack of 24)
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Customer Questions & Answers
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Question:
What is the purpose of the UFC901024 Regenerated Cellulose Amicon Ultra Centrifugal Filter Unit?
Answer: The UFC901024 is designed for concentration and purification of biomolecules. It effectively separates proteins, nucleic acids, and other macromolecules by size exclusion, allowing for efficient processing of samples in laboratory settings. Utilizing the PLGC Ultracel-10 membrane, this filter unit ensures high recovery rates and concentration efficiency, crucial for experiments that rely on accurate data collection and analysis. For example, researchers can use it to concentrate cell culture media or for buffer exchange in protein purification applications. -
Question:
What is the membrane material used in the UFC901024 filter, and what are its benefits?
Answer: The UFC901024 features a PLGC Ultracel-10 membrane made from regenerated cellulose. This material is known for its chemical compatibility, allowing it to be used with various solvents and buffers. Furthermore, the regenerated cellulose membrane offers high permeability and low protein binding, which ensures that the target biomolecules remain unaltered and retain their functionality during the filtration process. This quality makes the UFC901024 ideal for sensitive applications like enzyme assays or biomolecule studies. -
Question:
What is the significance of the 10kDa NMWL in the UFC901024 filter?
Answer: The 10kDa nominal molecular weight limit (NMWL) indicates that the UFC901024 filter unit is designed to retain molecules larger than 10 kilodaltons while allowing smaller molecules to pass through. This feature is essential for separating proteins from smaller contaminants, such as salts or metabolites. For instance, if you're purifying a protein, you can use this filter to concentrate your sample while removing unwanted small molecules, facilitating cleaner downstream analysis or experiments. -
Question:
How much sample can the UFC901024 filter hold?
Answer: The UFC901024 filter unit has a capacity of 15mL, making it suitable for a variety of laboratory applications involving different sample sizes. This volume allows researchers to work with substantial quantities of biomolecules for concentration or purification processes. For example, if you're handling cell lysates or media, this 15mL capacity is optimal for processing samples effectively without the need for multiple filtering steps. -
Question:
What is the diameter of the UFC901024 filter, and why does it matter?
Answer: The UFC901024 has a diameter of 29.7mm, which is standard for many laboratory centrifugal filters. This size allows it to fit into most standard centrifuge rotors, ensuring compatibility with common laboratory equipment. Selecting the right diameter is vital for effective centrifugation, allowing the filter to achieve optimal performance and reduce the risk of sample losses. This convenience means you can integrate it seamlessly into your existing workflows without needing special equipment. -
Question:
Can the UFC901024 filter be reused, and how should it be cleaned?
Answer: The UFC901024 is intended for single-use to prevent cross-contamination and maintain the integrity of samples. Once used, it should not be cleaned and reused, as this could compromise the results of your experiments. However, for non-critical applications where the risk is acceptable, some users may attempt to wash it gently with compatible solutions. In general, using it as directed ensures consistent results and reliable performance for sensitive analytical work. -
Question:
What types of applications are ideal for the UFC901024 filter?
Answer: The UFC901024 filter is ideal for applications such as protein concentration, buffer exchange, desalting, and purification of nucleic acids. Its design and membrane characteristics make it particularly suitable for molecular biology studies, including purifying plasmid DNA or concentrating antibody solutions. In lab scenarios, researchers frequently use this filter to prepare samples for SDS-PAGE or mass spectrometry, ensuring that the final preparations maintain the desired purity and concentration levels. -
Question:
Is the UFC901024 filter suitable for small-scale or large-scale experiments?
Answer: The UFC901024 is best suited for small-scale experiments due to its 15mL capacity. Researchers often use it for preliminary studies or pilot experiments before scaling up to larger volumes. Its ease of use and effective filtering makes it a practical choice for testing protocols or optimizing conditions before moving to larger filtration systems or setups. In scenarios where large sample volumes are necessary, combining multiple units could achieve the desired outcome while maintaining high-quality results. -
Question:
What precautions should I take when using the UFC901024 filter?
Answer: When using the UFC901024, it’s essential to handle the unit carefully to prevent contamination and ensure accurate results. Use aseptic techniques, work in a clean environment, and avoid touching the membrane surface. Additionally, ensure that your sample is compatible with the regenerated cellulose material to avoid chemical interactions that could impact the outcome. For instance, avoid using organic solvents that exceed the membrane’s compatible range. Following these precautions will help maintain the performance and integrity of your experiments. -
Question:
Where can I buy the UFC901024 Regenerated Cellulose Amicon Ultra Centrifugal Filter Unit?
Answer: You can purchase the UFC901024 Regenerated Cellulose Amicon Ultra Centrifugal Filter Unit at Ubuy in Malta. Ubuy offers a wide selection of laboratory supplies and ensures a smooth purchasing experience. By browsing Ubuy, you gain access to reliable product information and customer support to assist you in making the best choice for your laboratory needs.
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€ 309
Order now and get it around Friday, July 17
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Features & Benefits
- Ultra-fast spin times (as fast as 10-15 minutes)
- Heat-sealed membrane minimizes downstream extractables
- Translucent housing and volume gradations for convenient sample monitoring
- Direct pipette sample access eliminates processing step to recover concentrate
- High concentration factors of 80-100X
- Applications include protein purification, western blotting and protein extraction
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