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Item #:
124928214
TBST (TBS-Tween 20), 10X, pH 7.6, 1000ml/Unit
Item #:
124928214
€ 91
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Order now and get it around Friday, July 03
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Gentrop Liquid Buffers are perfect for molecular biology research with unparalleled purity and quality.
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What Stands Out
High Purity
The TBS-Tween 20 formulation ensures high purity and effectiveness, making it ideal for biological applications and reliable experimental results.
Optimal pH
Maintaining a pH of 7.6 ensures compatibility with biological samples, preventing degradation and ensuring the integrity of sensitive experiments.
Large Volume
Offered in a convenient 1000ml unit, this product provides excellent value and minimizes the need for frequent reordering, perfect for laboratories with high usage rates.
Product Details
| Item Weight | 2.2 lbs (1 kg) |
Who Should Buy?
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Laboratory Researchers
Ideal for scientists conducting experiments requiring precise pH conditions and stable formulations using surfactants like Tween 20.
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Biotechnology Applications
Suitable for biotechnologists working on protein stabilization and enzymatic reactions requiring gentle surfactants and buffering capacity.
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Pharmaceutical Formulators
Beneficial for formulators in the pharmaceutical industry aiming to create optimal drug delivery systems using emulsifying agents.
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Home Use
Not suitable for general household applications, as it is primarily designed for laboratory and industrial environments.
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Non-Scientific Fields
Users in non-scientific fields may find this product unnecessary and overly complex for everyday usage.
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Food Industry
Not recommended for food applications due to the specific formulation risks and lack of food-grade certification.
Product Description
TBST (TBS-Tween 20), 10X, pH 7.6, 1000ml/Unit
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Customer Questions & Answers
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Question:
What is TBST (TBS-Tween 20) used for?
Answer: TBST (TBS-Tween 20) is a buffer solution commonly used in molecular biology applications, particularly in Western blotting and immunoassays. It functions to maintain a stable pH, ensuring accurate results during experimental procedures. The inclusion of Tween 20 helps reduce non-specific binding, enhancing the specificity of detection methods. This application is crucial for obtaining clearer and more reliable results in protein analysis and quantification. -
Question:
What does the 10X concentration mean in TBST?
Answer: The 10X concentration indicates that TBST is ten times more concentrated than the working solution usually required for experiments. To prepare a working solution, you need to dilute it to 1X with distilled water. This allows for flexibility in preparing the exact amount needed for your experiments while reducing the risk of contamination from multiple preparation steps. For example, when using TBST in Western blots, it’s common to mix 100ml of 10X TBST with 900ml of water to achieve the 1X working solution. -
Question:
What is the significance of a pH of 7.6 in TBST?
Answer: A pH of 7.6 in TBST reflects a mildly alkaline buffer environment, which is important for maintaining the stability of proteins during assays. This pH level helps to mimic physiological conditions, ensuring that proteins maintain their functional structure. The optimal pH aids in achieving reliable interactions between the antibodies and antigens in immunological assays, leading to better assay performance and clearer results. -
Question:
Can TBST be stored for long periods?
Answer: TBST can be stored for several months if kept in a cool, dark place and appropriately sealed to prevent contamination. To maximize its shelf life, avoid repeated freeze-thaw cycles and store it in sterile containers. Proper storage ensures that the integrity and efficacy remain intact, which is essential for sensitive applications such as Western blotting or enzyme-linked immunosorbent assays (ELISA). Always check for any clarifying or precipitate before use, as these can indicate degradation. -
Question:
Why is Tween 20 added to TBST?
Answer: Tween 20 is a non-ionic detergent included in TBST to minimize non-specific binding of proteins to surfaces and to other proteins. This reduction in background noise is critical in assays such as Western blots, where specific detection is paramount. Its surfactant properties enhance the solubility of proteins, promoting clearer results in imaging and quantification. For example, when used in Western blotting, it improves the clarity of the bands, making analysis more straightforward. -
Question:
How do I prepare a 1X working solution from TBST?
Answer: To prepare a 1X working solution from TBST, mix 1 part of the 10X TBST solution with 9 parts of distilled water. For instance, if you need 100ml of 1X TBST, combine 10ml of 10X TBST with 90ml of distilled water. This dilution process is crucial for ensuring that the buffer maintains the appropriate concentration for experimental applications, such as washing steps in protein assays, leading to optimal results in your procedures. -
Question:
What are some common applications of TBST in the lab?
Answer: TBST is widely utilized in various laboratory applications, the most notable being Western blotting, where it serves as a washing buffer to remove unbound antibodies. It is also used in ELISAs for diluting samples and antibodies to enhance binding specificity. Additionally, TBST is applicable in immunofluorescence assays, aiding in the visualization of protein interactions. Its versatility makes it indispensable for researchers conducting protein analyses and various biochemical experiments. -
Question:
Is TBST suitable for all proteins in assays?
Answer: While TBST is effective for a wide range of proteins in assays, suitability can depend on the specific properties of the protein being studied. For example, some highly hydrophobic proteins might require alternative detergents for optimal solubility. Additionally, specific assays may have unique requirements regarding buffer composition. Therefore, it is recommended to assess your target protein characteristics and consult applicable protocols for tailored buffer needs. -
Question:
Where can I buy TBST (TBS-Tween 20), 10X, pH 7.6, 1000ml/Unit?
Answer: You can purchase TBST (TBS-Tween 20) 10X from Ubuy in Malta. Ubuy is known for offering a wide range of laboratory supplies and reagents, including TBST, ensuring reliable sourcing for your research needs. With Ubuy, you can easily order this essential buffer solution and have it delivered directly to your location, simplifying your lab supply management.
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€ 91
Order now and get it around Friday, July 03
This item is not restrict in my country.(Please click on above link if this item is not restrict in your country, So our team will review and allow.)
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Secured transaction
Ubuy works hard to protect your security and privacy. Our advanced payment security system ensures confidentiality by encrypting your information during transmission using AES (Advanced Encryption Standards) and SSL (Secure Socket Layer) protocols. Your payment details are 100% secure as we do not share your payment details with third party sellers.
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Features & Benefits
- Ultra pure, cGMP grade chemicals ensure the highest quality.
- Liquid buffers are essential for protein research and characterization.
- Formulated with 0.050 M Tris, 0.138 M NaCl, and 0.1% Tween-20.
- Manufactured using ultra pure, 4 fold purified water.
- Contains no RNase, DNase, endonucleases, or endotoxins.
- Molecular/Proteomic grade with > 99% purity.